Metabolism of pentachlorophenol in cell suspension cultures of wheat (Triticum aestivum L. ). Tetrachlorocatechol as a primary metabolite

Journal Article · · J. Agric. Food Chem.; (United States)

DOI:https://doi.org/10.1021/jf00080a032· OSTI ID:6904925

Schaefer, W; Sandermann, H Jr

Wheat cell suspension cultures were incubated with (U-/sup 14/C) pentachlorophenol (PCP; 1 ppm, 48 h, 27/sup 0/C). Soluble metabolites were formed in approx. 50% yield, another approx. 31% of the applied radioactivity being incorporated into the insoluble residue. The soluble metabolite fraction, and its ..beta..-D-glucoside conjugate components, the total insoluble residue, and its lignin components, were all found to contain a novel polar PCP derivative besides smaller amounts of tetrachlorohydroquinone and PCP. The novel derivative also predominated in intact wheat plants and was identified as tetrachlorocatechol by TLC, HPLC, GC, and EI as well as CI mass spectroscopy. Tetrachlorocatechol is a potential mutagen, so that the soluble and insoluble conjugates formed in wheat from PCP may present a toxicological hazard.

• Research Organization: Institut fuer Biochemische Pflanzenpathologie, Neuherberg (West Germany)
• OSTI ID: 6904925
• Journal Information: J. Agric. Food Chem.; (United States), Journal Name: J. Agric. Food Chem.; (United States) Vol. 36:2; ISSN JAFCA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AROMATICS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CULTURES
CEREALS
CHLORINATED AROMATIC HYDROCARBONS
DEVELOPERS
GRASS
HALOGENATED AROMATIC HYDROCARBONS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIGNIN
METABOLISM
METABOLITES
MUTAGENS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PESTICIDES
PHENOLS
PLANT CELLS
PLANTS
POLYPHENOLS
POLYSACCHARIDES
PYROCATECHOL
SACCHARIDES
TRACER TECHNIQUES
WHEAT