Detection of pentachlorophenol and its glucuronide and sulfate conjugates in fish bile and exposure water

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · J. Environ. Sci. Health, Part B; (United States)

DOI:https://doi.org/10.1080/03601238809372611· OSTI ID:6608147

Stehly, G R; Hayton, W L

The glucuronide and sulfate conjugates of pentachlorophenol (PCP) that were present in the bile and exposure water of goldfish (Carassius auratus) were used to develop methodology to quantify PCP and its metabolites. Reverse phase HPLC with radioactivity detection separated PCP and its metabolites, and was used to verify a method of quantification that used differential extraction and scintillation counting. Extractions of aqueous phase at pH 2 or 8, with butanol, ethyl acetate, or ether indicated that ether at pH 8 best separated PCP from its metabolites. The sulfate conjugate of PCP was the major metabolite produced when goldfish were exposed to 125 micrograms UC-PCP/l. It was present primarily in the exposure water, but also appeared in the bile.

• Research Organization: State Univ., Pullman, WA (USA)
• OSTI ID: 6608147
• Journal Information: J. Environ. Sci. Health, Part B; (United States), Journal Name: J. Environ. Sci. Health, Part B; (United States) Vol. 23:4; ISSN JPFCD
• Country of Publication: United States
• Language: English

Related Subjects

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560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BILE
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBON 14 COMPOUNDS
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
CONTAMINATION
COUNTING TECHNIQUES
EXTRACTION
FISHES
GOLDFISH
HALOGENATED AROMATIC HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
METABOLISM
METABOLITES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
SCINTILLATION COUNTING
SEPARATION PROCESSES
SOLVENT EXTRACTION
SULFATES
SULFUR COMPOUNDS
TRACER TECHNIQUES
VERTEBRATES
WATER POLLUTION