Analysis for nonextractable (bound) residues of pentachlorophenol in plant cells using a cell wall fractionation procedure
Journal Article
·
· Ecotoxicol. Environ. Saf.; (United States)
When plant cell cultures or aseptically grown wheat plants were treated with (/sup 14/C)-pentachlorophenol (PCP) a major part of the label was found in a nonextractable or bound residue fraction. Soluble polar conjugates participated in the formation of these residues which were mainly located in the plant cell walls. By a sequential fractionation procedure using enzymatic and chemical methods, 90 to 95% of the bound radioactivity could be attributed to individual cell wall components. The /sup 14/C label from PCP was found mainly in hemicellulose, lignin, and protein fractions. Associations of hemicellulose with PCP derivatives with molecular weights up to 500,000 were purified to constant specific radioactivity. Hydrolysis of this fraction released 32% PCP and other unidentified products.
- Research Organization:
- Institut fuer Pflanzenernaehrung und Bodenkunde, Braunschweig, Germany, F.R.
- OSTI ID:
- 5632113
- Journal Information:
- Ecotoxicol. Environ. Saf.; (United States), Journal Name: Ecotoxicol. Environ. Saf.; (United States) Vol. 2; ISSN EESAD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADSORPTION
AROMATICS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL CULTURES
CELL WALL
CEREALS
CHEMICAL ANALYSIS
CHLORINATED AROMATIC HYDROCARBONS
DISTRIBUTION
FOOD
GRASS
HALOGENATED AROMATIC HYDROCARBONS
LABELLED COMPOUNDS
LIGNIN
MOLECULAR WEIGHT
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVENTS
SORPTION
SOYBEANS
TISSUE DISTRIBUTION
UPTAKE
VEGETABLES
WHEAT
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADSORPTION
AROMATICS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL CULTURES
CELL WALL
CEREALS
CHEMICAL ANALYSIS
CHLORINATED AROMATIC HYDROCARBONS
DISTRIBUTION
FOOD
GRASS
HALOGENATED AROMATIC HYDROCARBONS
LABELLED COMPOUNDS
LIGNIN
MOLECULAR WEIGHT
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVENTS
SORPTION
SOYBEANS
TISSUE DISTRIBUTION
UPTAKE
VEGETABLES
WHEAT