Fragmentation, labeling and biodistribution studies of KS1/4, a monoclonal antibody
In this study, an IgG2a (KS1/4), a monoclonal antibody (MoAb) specific against a human lung adenocarcinoma (UCLA P-3) was successfully fragmented enzymatically to yield F(ab')/sub 2/ and Fab by using pepsin and papain, respectively. The kinetic of fragmentation of the MoAb was compared to that of human immunoglobulin G (IgG). A similar pattern of fragmentation was observed with both antibodies with a higher percentage yield of the F(ab')/sub 2/ and Fab obtained upon the fragmentation of the IgG by the enzymes. The KS1/4 and the two fragments were labeled with three different radionuclides, namely iodine-131, indium-111 and selenium-75. The radioiodination of the MoAb and the fragments was carried out by using a modified chloramine-T method. Radiometal labeling of the MoAb and the fragments with indium-111 was performed by using DTPA as a bifunctional chelating agent, while intrinsic labeling of the MoAb was done by culturing the hybridoma in the presence of /sup 75/Se-methionine. The biodistribution of the radiolabeled MoAb, F(ab')/sub 2/ and Fab fragments were performed by injecting the preparations intravenously into nude mice bearing human lung adenocarcinoma.
- Research Organization:
- University of Southern California, Los Angeles (USA)
- OSTI ID:
- 6902226
- Resource Relation:
- Other Information: Thesis (Ph. D.). Univ. of Southern California, Doheny Library, Micrographics, Dept., Los Angeles, CA 90089-0182
- Country of Publication:
- United States
- Language:
- English
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LUNGS
CARCINOMAS
MONOCLONAL ANTIBODIES
FRACTIONATION
LABELLING
TISSUE DISTRIBUTION
CHELATING AGENTS
HYBRIDOMAS
INDIUM 111
IODINE 131
MAN
METHIONINE
MICE
SELENIUM 75
TRACER TECHNIQUES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPOTROPIC FACTORS
MAMMALS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PRIMATES
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SELENIUM ISOTOPES
SEPARATION PROCESSES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550601 - Medicine- Unsealed Radionuclides in Diagnostics
550604 - Medicine- Unsealed Radionuclides in Therapy- (1980-)