Radioimmunoimaging of human lymphomas with I-131 tumor-specific monoclonal antibody

Conference · Sun Jan 01 04:00:00 EST 1984 · J. Nucl. Med.; (United States)

OSTI ID:6844433

Zimmer, A M; Epstein, A L; Spies, S M

The purpose of this study was to radiolabel an IgG2a monoclonal antibody (Lym-1) and fragments (Fab and F(ab')2) directed against human lymphomas (Raji) and to determine the biodistribution and feasibility of radioimmunoimaging. Radiolabeling with I-131 was achieved using Iodogen to which the monoclonal antibody (MA) and NaI-131 were added. Radioimmunoreactivity was performed utilizing a live cell assay of lymphoma cells (Raji). Athymic nude mice, each bearing a right thigh human lymphoma (Raji), were injected with 150-300 ..mu..Ci of I-131 labeled Ma, including Fab and F(ab')2 fragments, imaged up to 7 days after injection, sacrificed, and organ biodistribution performed. Results of the study demonstrated significant loss of immunoreactivity with the radioiodinated Fab fragments (11% binding) as opposed to F(ab')2 fragments (61% binding) or the whole antibody (65% binding). Highest tumor uptake was observed for the whole I-131 labeled antibody (8.2%) followed by F(ab')2 fragments (4.4%) and Fab fragments (0.9%). The most rapid whole body excretion was observed for radioiodinated Fab fragments followed by F(ab')2 fragments and whole antibody. Optimum tumor visualization for the radioiodinated F(ab')2 fragments and whole antibody was observed at 3 and 7 days after injection, with tumor/whole body ratios of 0.65 and 0.60 for F(ab')2 fragments and whole antibody, respectively. Biodistribution data obtained 7 days after injection confirmed high tumor uptake and low soft tissue distribution with tumor/liver ratios of 20.3 and 30.1 for the radioiodinated whole antibody and F(ab')2 fragments, respectively.

• Research Organization: Northwestern Univ. Medical School, Chicago, IL
• OSTI ID: 6844433
• Report Number(s): CONF-840619-
• Conference Information: Journal Name: J. Nucl. Med.; (United States) Journal Volume: 25:5
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL COMPOUNDS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DIAGNOSTIC USES
DISEASES
FEASIBILITY STUDIES
HALIDES
HALOGEN COMPOUNDS
HUMAN POPULATIONS
IMAGE PROCESSING
IMMUNOASSAY
IMMUNOLOGY
INJECTION
INORGANIC PHOSPHORS
INTAKE
INTERMEDIATE MASS NUCLEI
IODIDES
IODINE 131
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LYMPHOMAS
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
PHOSPHORS
POPULATIONS
PROCESSING
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
SODIUM COMPOUNDS
SODIUM IODIDES
TRACER TECHNIQUES
UPTAKE
USES
VERTEBRATES