In vivo kinetics of canine leukocytes labeled with technetium-99m HM-PAO and indium-111 tropolonate

Journal Article · Fri Jul 01 00:00:00 EDT 1988 · J. Nucl. Med.; (United States)

OSTI ID:6901412

Mock, B H; Schauwecker, D S; English, D; Young, K A; Wellman, H N

Two weeks after the introduction of osteomyelitis in three dogs, autologous leukocytes were dual-labeled with both (/sup 99m/Tc)HM-PAO and (/sup 111/In)tropolonate, and reinjected. Blood sampling and imaging were then performed. Two weeks later, the same dogs received simultaneous injections of singly-labeled (/sup 99m/Tc)WBC and (/sup 111/In)WBC for comparison. For both studies, blood samples were drawn over 6 hr to determine the respective blood clearance half-time (TB) and % recovery (%R0) of cell-bound radioactivity. There were no significant differences in the average TB results of the /sup 99m/Tc and /sup 111/In groups, either within or between the dual- and singly-labeled studies. The %R0 of singly-labeled (/sup 99m/Tc)WBC was about half that of the other groups (p less than 0.01); however, this difference was attributed to the dissimilar radiochemical purity of the (/sup 99m/Tc)HM-PAO reagents. Region of interest analysis of the 6 and 24 hr images revealed no significant differences between either cell label in the relative or absolute in vivo uptake at known sites of osteomyelitis, noninfected surgery, and normal bone marrow.

• Research Organization: Indiana Univ. Medical Center, Indianapolis (USA)
• OSTI ID: 6901412
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 29:7; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD-PLASMA CLEARANCE
BODY FLUIDS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
DOGS
DOUBLE LABELLING
ELECTRON CAPTURE RADIOISOTOPES
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
IMAGE PROCESSING
IN VIVO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLING
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOMETALLIC COMPOUNDS
OSTEOMYELITIS
OXIMES
PROCESSING
RADIOISOTOPES
SKELETAL DISEASES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES