Early increase in phosphatidyl choline synthesis by choline and transmethylation pathways in spreading fibroblasts

Journal Article · Sat Nov 01 00:00:00 EST 1986 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(86)90222-3· OSTI ID:6898694

Maziere, C; Maziere, J C; Mora, L; Polonovski, J

Phosphatidyl choline (PC) synthesis in trypsinized and reattaching fibroblasts during the spreading state was studied by incorporation of (/sup 14/C)choline and (methyl-/sup 14/C)methionine. The choline and phosphatidyl-ethanolamine (PE) transmethylation pathways were both transiently increased about 2-fold during the first 2 h after replating. Maximum increase appeared to be simultaneous with maximum spreading. Incorporation of (/sup 32/P)orthophosphate showed that the increase in PC synthesis was specific and most probably related to establishment of cell-substrate adhesion sites.

• Research Organization: CNRS, Paris, France
• OSTI ID: 6898694
• Journal Information: Exp. Cell Res.; (United States), Vol. 167:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
FIBROBLASTS
BIOLOGICAL PATHWAYS
PHOSPHOLIPIDS
BIOSYNTHESIS
PHOSPHORUS 32
CHOLINE
METHIONINE
METHYLATION
MORPHOLOGY
PHOSPHATES
ALCOHOLS
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ESTERS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPIDS
LIPOTROPIC FACTORS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
SOMATIC CELLS
SYNTHESIS
550301* - Cytology- Tracer Techniques