Enhanced incorporation of fatty acid into phosphatidyl choline that parallels histamine discharge in mast cells

Journal Article · Sun Jan 01 04:00:00 EST 1984 · J. Membr. Biol.; (United States)

DOI:https://doi.org/10.1007/BF01871061· OSTI ID:6047445

Castle, J D; Castle, A M; Ma, A K; Stukenbrok, H

Purified rat peritoneal and pleural mast cells preincubated briefly with radioactively labeled fatty acid were treated with A23187, which bypasses primary receptors in stimulating exocytosis. An enhanced incorporation of fatty acid into phosphatidyl choline (PC) that occurred in parallel with histamine release at 24-25 degrees C was observed and was initially proportional to the total amount of histamine discharged. Enhanced PC labeling and histamine secretion were also proportional at temperatures ranging from 17-37 degrees C. Both radioactive linoleic and palmitic acids were incorporated selectively at the beta-position of the glycerol backbone of PC. PC labeling by (3H)choline was not detectably different in control and stimulated cells, and phosphatidic acid did not exhibit selectively enhanced beta-acylation. Thus, the stimulated labeling in A23187-treated cells may occur secondary to the action of a phospholipase A2 that favors PC as a substrate. Other peritoneal cell types exhibit a very similar A23187-stimulat

• Research Organization: Yale Univ. School of Medicine, New Haven, CT
• OSTI ID: 6047445
• Journal Information: J. Membr. Biol.; (United States), Journal Name: J. Membr. Biol.; (United States) Vol. 79:3; ISSN JMBBB
• Country of Publication: United States
• Language: English

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