Active suppression of major histocompatibility complex class II gene expression during differentiation from B cells to plasma cells

Journal Article · Fri Apr 01 04:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.7.2229· OSTI ID:6896743

Latron, F; Maffei, A; Scarpellino, L; Bernard, M; Accolla, R S ^[1]; Jotterand-Bellomo, M ^[2]; Strominger, J L ^[3]

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1. Ludwig Institute for Cancer Research, Epalinges (Switzerland)
2. Centre Hospitalier Universitaire Vaudois, Lausanne (Switzerland)
3. Harvard Univ., Cambridge, MA (USA)

Constitutive expression of major histocompatibility complex class II genes is acquired very early in B-cell ontogeny and is maintained up to the B-cell blast stage. Terminal differentiation in plasma cells is, however, accompanied by a loss of class II gene expression. In B cells this gene system is under the control of several loci encoding transacting factors with activator function, one of which, the aIr-1 gene product, operates across species barriers. In this report human class II gene expression is shown to be extinguished in somatic cell hybrids between the human class II-positive B-cell line Raji and the mouse class-II negative plasmacytoma cell line P3-U1. Since all murine chromosomes are retained in these hybrids and no preferential segregation of a specific human chromosome is observed, the results are compatible with the presence of suppressor factors of mouse origin, operating across species barriers and inhibiting class II gene expression. Suppression seems to act at the level of transcription or accumulation of class II-specific mRNA, since no human, and very few murine, class II transcripts are detectable in the hybrids.

• OSTI ID: 6896743
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 85:7; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CARBOHYDRATES
CELL DIFFERENTIATION
CELL FLOW SYSTEMS
CHROMOSOMES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
GENE REGULATION
GLUCOPROTEINS
GLYCOPROTEINS
HYBRIDIZATION
IMMUNITY
ISOTOPES
KARYOTYPE
LEUKOCYTES
LIGHT NUCLEI
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MESSENGER-RNA
MICE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMA CELLS
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SACCHARIDES
SOMATIC CELLS
TRANSCRIPTION
VERTEBRATES