Human chromosome 16 encodes a factor involved in induction of class II major histocompatibility antigens by interferon. gamma

Journal Article · Mon Jul 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.14.6077· OSTI ID:5933057

Bono, M R; Alcaide-Loridan, C; Couillin, P; Letouze, B; Grisard, M C; Fellous, M ^[1]; Jouin, H ^[2]

---

1. Inst. National de la Sante et de la Recherche Medicale, Paris (France)
2. Centre National de la Recherche Scientifique, Paris (France)

Interferon {gamma} (IFN-{gamma}) induces expression of class II major histocompatibility complex (MHC)-encoded antigens in immunocompetent cells. To gain further insight into the mechanism of this induction, the authors preparded somatic cell hybrids between different human cell lines and a murine cell line, RAG, that does not express murine class II MHC antigens before or after treatment with murine IFN-{gamma}. Some of the resulting cell hybrids express murine class II MHC antigens when treated with murine IFN-{gamma}. This inducible phenotype is correlated with the presence of human chromospme 16. It has been shown previously that the induction of class I MHC antigens by human IFN-{gamma} in human-rodent hybrids requires the presence of species-specific factors encoded by chromosome 6, which bears the gene for the human IFN-{gamma} receptor, and chromosome 21, whose product(s) is necessary for the transduction of human IFN-{gamma} signals. In this report, they show that the induction of murine class II MHC antigens by human IFN-{gamma} in the human-RAG cell hybrids requires, likewise, the presence of human chromosomes 6 and 21, in addition to chromosome 16. In some of these hybridsm when all three of these human chromosomes were present, induction of cell-surface HLA-DR antigens was also observed. The results demonstrate that human chromosome 16 encodes a non-species-specific factor involved in the induction of class II MHC antigens by IFN-{gamma}.

• OSTI ID: 5933057
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:14; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
ANTIGENS
BIOLOGICAL EFFECTS
CHROMOSOMES
GENETIC MAPPING
GROWTH FACTORS
HISTOCOMPATIBILITY COMPLEX
HUMAN CHROMOSOME 16
HUMAN CHROMOSOME 21
HUMAN CHROMOSOMES
INTERFERON
LYMPHOKINES
MAPPING
MITOGENS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PROTEINS