Trichloroethylene biodegradation by a methane-oxidizing bacterium
Journal Article
·
· Appl. Environ. Microbiol.; (United States)
OSTI ID:6895382
Trichloroethylene (TCE), a common ground water contaminant, is a suspected carcinogen that is highly resistant to aerobic biodegradation. An aerobic, methane-oxidizing bacterium was isolated that degrades TCE in pure culture at concentrations commonly observed in contaminated ground water. Strain 46-1, a type I methanotrophic bacterium, degraded TCE if grown on methane or methanol, producing CO/sub 2/ and water-soluble products. Gas chromatography and /sup 14/C radiotracer techniques were used to determine the rate, methane dependence, and mechanism of TCE biodegradation. TCE biodegradation by strain 46-1 appears to be a cometabolic process that occurs when the organism is actively metabolizing a suitable growth substrate such as methane or methanol. It is proposed that TCE biodegradation by methanotrophs occurs by formation of TCE epoxide, which breaks down spontaneously in water to form dichloroacetic and glyoxylic acids and one-carbon products.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- AC05-84OR21400; FG03-85ER13306
- OSTI ID:
- 6895382
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 54:4; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
Similar Records
Bacteria that degrade hazardous waste: The isolation of trichloroethylene-degrading methanotrophic bacteria and development of monoclonal antibodies specific to them
Influence of the endogenous storage lipid poly-[beta]-hydroxybutyrate on the reducing power availability during cometabolism of trichloroethylene and naphthalene by resting methanotrophic mixed cultures
Biodegradation of chlorinated ethenes by a methane-utilizing mixed culture
Thesis/Dissertation
·
Thu Dec 31 23:00:00 EST 1987
·
OSTI ID:6969364
Influence of the endogenous storage lipid poly-[beta]-hydroxybutyrate on the reducing power availability during cometabolism of trichloroethylene and naphthalene by resting methanotrophic mixed cultures
Journal Article
·
Sat May 01 00:00:00 EDT 1993
· Applied and Environmental Microbiology; (United States)
·
OSTI ID:6444956
Biodegradation of chlorinated ethenes by a methane-utilizing mixed culture
Journal Article
·
Mon Mar 31 23:00:00 EST 1986
· Appl. Environ. Microbiol.; (United States)
·
OSTI ID:5842906
Related Subjects
520200 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
BACTERIA
BIODEGRADATION
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METHANE
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
POLLUTION
TRACER TECHNIQUES
WATER POLLUTION
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
BACTERIA
BIODEGRADATION
CARBON 14 COMPOUNDS
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROCARBONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METABOLISM
METHANE
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
POLLUTION
TRACER TECHNIQUES
WATER POLLUTION