Influence of the endogenous storage lipid poly-[beta]-hydroxybutyrate on the reducing power availability during cometabolism of trichloroethylene and naphthalene by resting methanotrophic mixed cultures

Journal Article · Sat May 01 00:00:00 EDT 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6444956

Henrysson, T; McCarty, P L ^[1]

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1. Stanford Univ., CA (United States)

Trichloroethylene (TCE) is one of the most frequently detected toxic contaminants in ground water. TCE degradation by methanotrophs is a cometabolic process in which methane, but not TCE, is used as the primary substrate for energy and cell carbon. Developing a bioreactor in which cells can degrade TCE without competition is of interest in in-situ treatments. This study determines what relationship might exist between the poly-[beta]-hydroxybutyrate (PHB) content in methanotrophic cells and their naphthalene oxidation rates. In addition, the relationship between TCE transformation rate and capacity and PHB content is examined. Finally, the possibilities for manipulation of the PHB content are examined, as is the effect of PHB on the observed decrease in methane monooxygenase activity with resting aerobic cells. 30 refs., 4 figs., 3 tabs.

• OSTI ID: 6444956
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 59:5; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKANES
ALKENES
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CARBON SOURCES
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
ETHYLENE
GROUND WATER
HYDROCARBONS
HYDROGEN COMPOUNDS
METABOLISM
METHANE
MICROORGANISMS
NAPHTHALENE
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTANTS
WATER