Bacteria that degrade hazardous waste: The isolation of trichloroethylene-degrading methanotrophic bacteria and development of monoclonal antibodies specific to them
Thesis/Dissertation
·
OSTI ID:6969364
Trichloroethylene (TCE), a suspected carcinogen, is one of the most frequently reported groundwater contaminants at hazardous waste sites in the US. An aerobic, methane-oxidizing bacterium was isolated that degrades TCE in pure culture at concentrations commonly observed in contaminated groundwater. Strain 46-1, a Type I methanotrophic bacterium, degraded TCE when growing on methane or methanol, producing CO{sub 2} and water-soluble products. Gas chromatography and {sup 14}C radiotracer techniques were used to determine the rate, methane dependence, and mechanism of TCE biodegradation. TCE biodegradation by strain 46-1 appears to be a co-metabolic process that occurs when the organism is actively metabolizing a suitable growth substrate such as methane or methanol. Five mouse monoclonal antibodies (MABS) that specifically bind strain 46-1 were prepared by conventional hybridoma technology. These MABS are apparently biochemically distinct and were used to develop enzyme-linked and fluorescent immunoassays to detect strain 46-1 cells in environmental samples. A fluorescent immunoassay utilizing four of these MABS easily distinguished laboratory-grown 46-1 cells from other methanotrophic and heterotrophic bacteria, but failed to detect 46-1 cells in groundwater samples and cultures.
- Research Organization:
- Florida State Univ., Tallahassee, FL (USA)
- OSTI ID:
- 6969364
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
54 ENVIRONMENTAL SCIENCES
540311* -- Environment
Aquatic-- Basic Studies-- Radiometric Techniques-- (1990-)
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKANES
ALKENES
ANIMALS
ANTIBODIES
BIOASSAY
BIODEGRADATION
CARBON 14 COMPOUNDS
CARCINOGENS
CHEMICAL REACTIONS
CHLORINE COMPOUNDS
DATA
DECOMPOSITION
ETHYLENE
EXPERIMENTAL DATA
GROUND WATER
HALOGEN COMPOUNDS
HAZARDOUS MATERIALS
HYDROCARBONS
HYDROGEN COMPOUNDS
IMMUNOASSAY
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METABOLISM
METHANE
MICE
MONOCLONAL ANTIBODIES
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
RODENTS
TRACER TECHNIQUES
VERTEBRATES
WATER
WATER POLLUTION
540311* -- Environment
Aquatic-- Basic Studies-- Radiometric Techniques-- (1990-)
550701 -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKANES
ALKENES
ANIMALS
ANTIBODIES
BIOASSAY
BIODEGRADATION
CARBON 14 COMPOUNDS
CARCINOGENS
CHEMICAL REACTIONS
CHLORINE COMPOUNDS
DATA
DECOMPOSITION
ETHYLENE
EXPERIMENTAL DATA
GROUND WATER
HALOGEN COMPOUNDS
HAZARDOUS MATERIALS
HYDROCARBONS
HYDROGEN COMPOUNDS
IMMUNOASSAY
INFORMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
METABOLISM
METHANE
MICE
MONOCLONAL ANTIBODIES
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
RODENTS
TRACER TECHNIQUES
VERTEBRATES
WATER
WATER POLLUTION