Chromosomal localization of a sequence with in vivo activity for initiation of DNA replication

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Somatic Cell and Molecular Genetics; (United States)

DOI:https://doi.org/10.1007/BF01233959· OSTI ID:6885886

Shihab-El-Deen, A; Wu, Cunle; Zannis-Hadjopoulos, M; Price, G B ^[1]; Hamerton, J L ^[2]

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1. McGill Univ., Montreal, Quebec (Canada)
2. Univ. of Manitoba, Winnipeg (Canada)

The genomic fragment containing the sequence of human cDNA clone 343, previously characterized as capable of autonomous replication upon transfection into mammalians cells and occupying a genomic region inclusive of an initiation zone for DNA replication, was mapped on human chromosome 6q-22qter by a combination of in situ hybridization and G-banding. Southern blot hybridization with a panel of human-hamster somatic cells confirmed the location of the 343 gene on chromosome 6. Fragile sites have been mapped to the region at 6q21 and 6q26. Several neoplastic disorders, including melanoma, acute nonlymphocytic leukemia, acute lymphocytic leukemia, and malignant lymphoma, have also exhibited translocations and deletions involving the region 6q21-6q27. 19 refs., 3 figs., 1 tab.

• OSTI ID: 6885886
• Journal Information: Somatic Cell and Molecular Genetics; (United States), Journal Name: Somatic Cell and Molecular Genetics; (United States) Vol. 19:1; ISSN 0740-7750; ISSN SCMGDN
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BANDING TECHNIQUES
CARCINOMAS
CHROMOSOMES
CYTOLOGICAL TECHNIQUES
DISEASES
DNA REPLICATION
EPITHELIOMAS
GENES
GENETIC MAPPING
HUMAN CHROMOSOMES
HYBRIDIZATION
IMMUNE SYSTEM DISEASES
LEUKEMIA
LYMPHOMAS
MAPPING
MELANOMAS
NEOPLASMS
NUCLEIC ACID REPLICATION
TRANSLOCATION