Inducible reactivation and mutagenesis of UV-irradiated bacteriophage P22 in Samonella typhimurium LT2 containing the plasmid pKM101

Journal Article · Tue Aug 01 04:00:00 EDT 1978 · J. Bacteriol.; (United States)

OSTI ID:6880301

Walker, G C

The inducible (Weigle) reactivation of uv-irradiated bacteriophage P22 has been examined in strains of Salmonella typhimurium with and without the mutagenesis-enhancing plasmid pKM101. A large inducible reactivation was observed in the plasmid-containing strain, but only a small response was observed in the strain lacking the plasmid. An increased frequency of clear-plaque mutants was detected among the survivors. The efficiencies of the plasmid-mediated and cellular repair processes have been determined. The kinetics of induction of the phage reactivation have been investigated. The relationship of the observed results to the inducible reactivation of uv-irradiated lambda in Escherichia coli and to error-prone repair is discussed.

• Research Organization: Univ. of California, Berkeley
• OSTI ID: 6880301
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 135:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CONSTITUENTS
ELECTROMAGNETIC RADIATION
MICROORGANISMS
MUTAGENESIS
PARASITES
PLASMIDS
RADIATION EFFECTS
RADIATIONS
SALMONELLA
SALMONELLA TYPHIMURIUM
ULTRAVIOLET RADIATION
VIRUSES