Alteration of bacteriophage attachment capacity by near-uv irradiation
- Univ. of Minnesota, St. Paul
Near-uv (NUV) (300 to 400 nm) and far-uv (FUV) (254 nm) radiations damage bacteriophage by different mechanisms. Host cell reactivation, Weigle reactivation, and multiplicity reactivation were observed upon FUV, but not upon NUV irradiation. Also, the number of his/sup +/ recombinants increased with P22 bacteriophage transduction in Salmonella typhimurium after FUV, but not after NUV irradiation. This loss of reactivation and recombination after NUV irradiation was not necessarily due to host incapability to repair phage damage. Instead, the phage genome failed to enter the host cell after NUV irradiation. In the case of NUV-irradiated T7 phage, this was determined by genetic crosses with amber mutants, which demonstrated that either ''all'' or ''none'' of a T7 genome entered the Escherichia coli cell after NUV treatment. Further studies with radioactively labeled phage indicated that irradiated phage failed to adsorb to host cells. This damage by NUV was compared with the protein-DNA cross-link observed previously, when phage particles were irradiated with NUV in the presence of H/sub 2/O/sub 2/. H/sub 2/O/sub 2/ (in nonlethal concentration) acts synergistically with NUV so that equivalent phage inactivation is achieved by much lower irradiation doses.
- OSTI ID:
- 6723060
- Journal Information:
- J. Virol.; (United States), Vol. 43:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIOPHAGES
INACTIVATION
FAR ULTRAVIOLET RADIATION
BIOLOGICAL RADIATION EFFECTS
NEAR ULTRAVIOLET RADIATION
COMPARATIVE EVALUATIONS
ESCHERICHIA COLI
HOST-CELL REACTIVATION
HYDROGEN PEROXIDE
RECOMBINANT DNA
SALMONELLA TYPHIMURIUM
SYNERGISM
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
ELECTROMAGNETIC RADIATION
HYDROGEN COMPOUNDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PARASITES
PEROXIDES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SALMONELLA
ULTRAVIOLET RADIATION
VIRUSES
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)