Translesion synthesis is the main component of SOS repair in bacteriophage lambda DNA

Journal Article · Fri Sep 01 04:00:00 EDT 1989 · Journal of Bacteriology; (USA)

OSTI ID:5348065

Defais, M; Lesca, C; Monsarrat, B; Hanawalt, P ^[1]

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1. Centre National de la Recherche Scientifique, Toulouse (France)

Agents that interfere with DNA replication in Escherichia coli induce physiological adaptations that increase the probability of survival after DNA damage and the frequency of mutants among the survivors (the SOS response). Such agents also increase the survival rate and mutation frequency of irradiated bacteriophage after infection of treated bacteria, a phenomenon known as Weigle reactivation. In UV-irradiated single-stranded DNA phage, Weigle reactivation is thought to occur via induced, error-prone replication through template lesions. Weigle reactivation occurs with higher efficiency in double-stranded DNA phages such as lambda, and we therefore asked if another process, recombination between partially replicated daughter molecules, plays a major role in this case. To distinguish between translesion synthesis and recombinational repair, we studied the early replication of UV-irradiated bacteriophage lambda in SOS-induced and uninduced bacteria. To avoid complications arising from excision of UV lesions, we used bacterial uvrA mutants, in which such excision does not occur. Our evidence suggests that translesion synthesis is the primary component of Weigle reactivation of lambda phage in the absence of excision repair. The greater efficiency in Weigle reactivation of double-stranded DNA phage could thus be attributed to some inducible excision repair unable to occur on single-stranded DNA. In addition, after irradiation, lambda phage replication seems to switch prematurely from the theta mode to the rolling circle mode.

• OSTI ID: 5348065
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 171:9; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMATOGRAPHY
DNA HYBRIDIZATION
DNA REPAIR
DNA REPLICATION
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYBRIDIZATION
LIQUID COLUMN CHROMATOGRAPHY
MICROORGANISMS
MOLECULAR WEIGHT
MUTATION FREQUENCY
NUCLEIC ACID REPLICATION
PARASITES
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SEPARATION PROCESSES
SURVIVAL TIME
ULTRAVIOLET RADIATION
VIRUSES