SOS repair can be about as effective for single-stranded DNA as for double-stranded DNA and even more so

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Journal of Bacteriology; (USA)

OSTI ID:6509490

Tessman, I ^[1]

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1. Purdue Univ., West Lafayette, IN (USA)

As ordinarily measured, the SOS repair of damaged DNA by Weigle reactivation appears to be more effective for double-stranded (ds) than for single-stranded (ss) DNA bacteriophages. A complicating feature, which is usually not considered, is the possibility of DNA-protein cross-linking of ssDNA to the viral capsid, which would conceivably be an extraneous source of nonreactivable lesions. This idea is supported in studies of phage S13 by the observation that photoreactivation more than doubles when naked ssDNA is substituted for encapsidated ssDNA as the UV target. The same effect was observed for Weigle reactivation; there was little, if any, difference in the reactivation of ssDNA and dsDNA when naked DNA was irradiated. Moreover, in a uvrA mutant, ssDNA actually had the advantage; Weigle reactivation was then more than twice as effective for ssDNA as for dsDNA. It is also shown that when a suitable measure of Weigle mutagenesis is used, there is no convincing evidence that dsDNA is mutagenized more effectively than ssDNA.

• OSTI ID: 6509490
• Journal Information: Journal of Bacteriology; (USA), Journal Name: Journal of Bacteriology; (USA) Vol. 172:9; ISSN JOBAA; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BACTERIOPHAGES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL REACTIONS
CROSS-LINKING
DNA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
MICROORGANISMS
MUTANTS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
POLYMERIZATION
RADIATION EFFECTS
RADIATION INDUCED MUTANTS
RADIATIONS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION
VIRUSES