Experimental analysis of embryogenesis of cerebellum in rat. II. Morphogenetic malformations following x-ray irradiation on day 18 of gestation

Journal Article · Thu Dec 01 04:00:00 EST 1977 · J. Comp. Neurol.; (United States)

DOI:https://doi.org/10.1002/cne.901760309· OSTI ID:6880142

Das, G D

Rat embryos of 18 days gestation were exposed in utero to 170 R of x-ray irradiation. Embryos were collected six hours, 1, 2, and 3 days after irradiation, and animals of 2-, 6-, 15- and 30-day-old postnatal age were sacrificed. Six hours after irradiation pyknosis of cells was noticed in the external granular layer along the posterior aspect of the cerebellum. Neuroblasts, destined to differentiate into Purkinje cells, were found arrested in their migratory path. During subsequent periods of embryogenesis the external granular layer was found recovered, and clustering of the neuroblasts were disorganized and fragmented. This abnormal clustering of neuroblasts was permanent, and the external granular layer followed the same abnormal pattern in its growth. During postnatal development the internal granular layer also was found to follow the abnormal pattern of Purkinje cell layer. Those abnormal developmental events were seen to lead to malformed folia in the anterior regions of the cerebellum. In addition to it the cerebellum of x-ray irradiated animals appeared smaller than the normal. Issues having a bearing on the differential radiosensitivity of different cells, factors determining the small size of the cerebellum, and cellular events determining the morphogenetic malformations are discussed.

• Research Organization: Purdue Univ., Lafayette, IN
• OSTI ID: 6880142
• Journal Information: J. Comp. Neurol.; (United States), Journal Name: J. Comp. Neurol.; (United States) Vol. 176:3; ISSN JCNEA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CEREBELLUM
ELECTROMAGNETIC RADIATION
EMBRYOS
IONIZING RADIATIONS
IRRADIATION
MAMMALS
MORPHOLOGICAL CHANGES
NEONATES
NERVOUS SYSTEM
ONTOGENESIS
ORGANS
PRENATAL IRRADIATION
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RATS
RODENTS
TIME DEPENDENCE
VERTEBRATES
X RADIATION