Monoclonal antibodies to human urinary thrombopoietin
Journal Article
·
· Proc. Soc. Exp. Biol. Med.; (United States)
Monoclonal antibodies (MA) to a thrombocytopoiesis-stimulating factor (TSF or thrombopoietin) were obtained from hybridomas derived from the fusion of P3 x 63/Ag 8 cells and spleen cells from TSF-immunized BALB/c mice. Media from several hybrid cultures were tested in a microantibody detection technique that measured the binding of MA to a /sup 125/I-purified TSF preparation from human embryonic kidney (HEK) cells. Hybridized cells were injected into pristane-primed mice and the antibodies produced in the ascites fluid were also shown to bind the /sup 125/I-TSF. Compared to the results of normal mouse serum, ascites fluid containing MA was shown to bind the unlabeled TSF from HEK cells. The TSF activity was significantly reduced in the supernatant fluid after precipitating the TSF-anti-TSF immune complex by a second antibody when tested in an immunothrombocythemic mouse assay. After SDS-PAGE, the precipitate from this TSF-Ma conjugate showed that the antiserum bound a single 32,000 mol wt component, indicating the monospecificity of the MA. MA directed toward human TSF will allow studies that were not previously possible.
- Research Organization:
- Univ. of Tennessee, Knoxville (USA)
- OSTI ID:
- 6879374
- Journal Information:
- Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 182; ISSN PSEBA
- Country of Publication:
- United States
- Language:
- English
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Wed Dec 31 23:00:00 EST 1975
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OSTI ID:7362339
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD FORMATION
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HYBRIDOMAS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RADIORECEPTOR ASSAY
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SULFUR 35
SULFUR ISOTOPES
THROMBOPOIESIS
TRACER TECHNIQUES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD FORMATION
CELL DIFFERENTIATION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HYBRIDOMAS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MICE
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RADIORECEPTOR ASSAY
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SULFUR 35
SULFUR ISOTOPES
THROMBOPOIESIS
TRACER TECHNIQUES
VERTEBRATES