Comparative studies on the lysosomal association of monomeric /sup 239/Pu and /sup 241/Am in rat and Chinese hamster liver: analysis with sucrose, metrizamide, and Percoll density gradients of subcellular binding as dependent on time

Journal Article · Tue May 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576237· OSTI ID:6878652

Suetterlin, U; Thies, W G; Haffner, H; Seidel, A

The binding of /sup 239/Pu and /sup 241/Am in the livers of Chinese hamsters and rats was analyzed by centrifugation of a mitochondrial-lysosomal fraction in sucrose, metrizamide, and Percoll density gradients at intervals between 4 and 70 days after nuclide injection. The behavior of /sup 239/Pu and /sup 241/Am during the centrifugation experiments was very similar. In contrast to the results for rats, the median densities of the nuclide fraction liberated by addition of Triton X-100, and the nuclide profiles do not respond typically to Triton WR 1339 treatment of the animals. It was concluded from the results that the major fraction of /sup 239/Pu and /sup 241/Am remained bound to typical lysosomes in rat liver, whereas those in hamster liver may be transformed into telolysosomes. Possibly, a vesicular biliary transport system for certain heavy metals, for which evidence exists in rat liver, does not occur in Chinese hamster liver.

• Research Organization: Kernforschungszentrum, Karlsruhe, West Germany
• OSTI ID: 6878652
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 98:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

551001 -- Physiological Systems-- Tracer Techniques
560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID PHOSPHATASE
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
AMERICIUM 241
AMERICIUM ISOTOPES
AMIDES
ANIMALS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CENTRIFUGATION
COMPARATIVE EVALUATIONS
CONTRAST MEDIA
DIGESTIVE SYSTEM
DISACCHARIDES
ENZYME ACTIVITY
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
GLANDS
HAMSTERS
HEAVY NUCLEI
HYDROLASES
ISOTOPES
LIVER
LYSOSOMES
MAMMALS
METRIZAMIDE
NUCLEI
ODD-EVEN NUCLEI
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHATASES
PLUTONIUM 239
PLUTONIUM ISOTOPES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
SACCHARIDES
SACCHAROSE
SEPARATION PROCESSES
TIME DEPENDENCE
VERTEBRATES
YEARS LIVING RADIOISOTOPES