Association of 239Pu with lysosomes in rat, Syrian hamster, and Chinese hamster liver as studied by carrier-free electrophoresis and electron microscopic autoradiography with 241Pu

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576615· OSTI ID:5801872

Seidel, A; Krueger, E W; Wiener, M; Hotz, G; Balani, M; Thies, W G

The binding of injected monomeric plutonium in the liver of rats, Syrian hamsters, and Chinese hamsters (species which show profound differences in their ability to eliminate /sup 239/Pu from the liver) was investigated by carrier-free electrophoresis using /sup 239/Pu and electron microscopic autoradiography with /sup 241/Pu. These studies are part of a program designed to obtain a better understanding of the mechanisms of the clearance of transuranium elements from liver of different mammals and man. Between 4 and 9 days after nuclide injection, a clear correlation between the majority of the /sup 239/Pu and lysosomal enzymes was observed when the mitochondrial-lysosomal (ML) fraction of the livers was analyzed by carrier-free electrophoresis. In the two hamster species, a second /sup 239/Pu peak exists from the beginning and increases with time to comprise 50% of the total radioactivity at later times. During electron microscopic examination 4 days after /sup 241/Pu injection, beta tracks were frequently observed over globular structures resembling dense bodies in Chinese hamster liver. They were also observed frequently over chromatin-rich portions of the cell nuclei. These results, together with those from previous density gradient studies, show that lysosomes are the primary deposition site for /sup 239/Pu in the liver cytoplasm of these three rodent species. The hypothesis of a morphologic transformation of these lysosomes with time in hamster liver and of rapid bulk exocytosis of the lysosomes in rats are still possible explanations for the extreme differences in the elimination among the three species.

• Research Organization: Institut fuer Genetik und fuer Toxikologie von Spaltstoffen, Germany, F.R.
• OSTI ID: 5801872
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States); ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BODY
CELL CONSTITUENTS
CLEARANCE
DIGESTIVE SYSTEM
ELECTRON MICROSCOPY
ELECTROPHORESIS
EVEN-ODD NUCLEI
GLANDS
HAMSTERS
HEAVY NUCLEI
ISOTOPES
LIVER
LYSOSOMES
MAMMALS
MICROSCOPY
NUCLEI
ORGANOIDS
ORGANS
PLUTONIUM 239
PLUTONIUM 241
PLUTONIUM ISOTOPES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES