Initial early distribution of monomeric /sup 239/Pu and /sup 241/Am in rat liver as determined by Triton WR 1339 injection

Journal Article · Sun Feb 01 04:00:00 EST 1981 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575570· OSTI ID:6566922

Gruner, R ^[1]; Seidel, A; Winter, R

---

1. Amersham Buchler GmbH and Co KG, Gieselweg, Germany

The subcellular distribution of intravenously injected monomeric /sup 239/Pu and /sup 241/Am in rat liver was investigated using the injection of the nonionic detergent Triton WR 1339 as a method for obtaining purified lysosomes. It has been shown that a considerable proportion of both nuclides moves to lighter densities under the influence of Triton WR 1339 with a corresponding shift of two lysosomal marker enzymes. These observations and the lack of correspondence between the radionuclide distribution profiles and the marker enzymes for other cell organelles have led to the conclusion that lysosomes are an important storage organelle for /sup 239/Pu and /sup 241/Am in rat liver at the sixth day after injection. There was also some indication that the radionuclides might be associated partly with /sup 59/Fe-binding components of the cell. However, the possibility of artifacts in this respect cannot be excluded. The use of Triton WR 1339 in subcellular binding studies causes several problems which need careful consideration.

• OSTI ID: 6566922
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 85:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID PHOSPHATASE
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ADDITIVES
ALPHA DECAY RADIOISOTOPES
AMERICIUM 241
AMERICIUM ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
CENTRIFUGATION
DAYS LIVING RADIOISOTOPES
DETERGENTS
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON MICROSCOPY
EMULSIFIERS
ENZYMES
ESTERASES
EVEN-ODD NUCLEI
GLANDS
HEAVY NUCLEI
HYDROLASES
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
LIVER
LYSOSOMES
MAMMALS
MICROSCOPY
NUCLEI
ODD-EVEN NUCLEI
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PHOSPHATASES
PLUTONIUM 239
PLUTONIUM ISOTOPES
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
SEPARATION PROCESSES
SURFACTANTS
TISSUE DISTRIBUTION
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
VERTEBRATES
WETTING AGENTS
YEARS LIVING RADIOISOTOPES