Ketone bodies and islet function: sup 45 Ca handling, insulin synthesis, and release

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · American Journal of Physiology; (USA)

OSTI ID:6875050

Malaisse, W J; Lebrun, P; Yaylali, B; Camara, J; Valverde, I; Sener, A ^[1]

---

1. Brussels Free Univ. (Belgium)

D-(-)-beta-Hydroxybutyrate and acetoacetate cause a rapid, sustained, and rapidly reversible stimulation of insulin release from rat pancreatic islets incubated in the presence, but not absence, of D-glucose. This coincides with stimulation of both proinsulin biosynthesis and {sup 45}Ca net uptake. The ketone bodies also decrease {sup 45}Ca outflow from prelabeled islets perifused in the absence of Ca{sup 2+} and, in contrast, enhance effluent radioactivity in the presence of Ca{sup 2+}. In the presence of D-glucose, the secretory response to D-(-)-beta-hydroxybutyrate is concentration related in the 2.5-20 mM range, abolished in the absence of Ca{sup 2+} or presence of KCN, and enhanced by theophylline and forskolin. It corresponds grossly to a shift to the left of the sigmoidal curve relating insulin output to the ambient concentration of D-glucose. The secretory, biosynthetic, and cationic response to acetoacetate is less marked than that evoked by an equimolar concentration of D-(-)-beta-hydroxybutyrate. These features are compatible with the view that the insulinotropic action of ketone bodies would be causally linked to their metabolism in islet cells.

• OSTI ID: 6875050
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 259; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETOACETATES
ALDEHYDES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
BODY
BUTYRIC ACID
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
FUNCTIONS
GLANDS
GLUCOSE
HEXOSES
HORMONES
IN VITRO
INSULIN
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
MEMBRANE TRANSPORT
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
PHENYLALANINE
RADIOISOTOPES
RATS
RODENTS
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES