Stimulus-secretion coupling of arginine-induced insulin release: Comparison with histidine-induced insulin release

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Endocrinology; (USA)

DOI:https://doi.org/10.1210/endo-127-1-107· OSTI ID:6611294

Sener, A; Blachier, F; Rasschaert, J; Malaisse, W J ^[1]

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1. Brussels Free Univ. (Belgium)

L-Histidine, when tested at a 10-mM concentration, caused a rapid and sustained stimulation of insulin release from rat islets exposed to either D-glucose (7.0 or 8.3 mM) or L-leucine (10.0 mM). The stimulation of insulin release could not be ascribed to an increase in oxygen uptake, to the generation of histamine from L-histidine, or to its participation in a transglutaminase-catalyzed reaction. Like other cationic amino acids, however, L-histidine rapidly accumulated in islet cells, increased 86Rb outflow from prelabeled islets perifused in the presence or absence of extracellular Ca2+, and stimulated the entry of Ca2+ into islet cells. Yet, the amount of exogenous L-histidine present in the islet cells with a positively charged side chain was estimated to be below the threshold value required for stimulation of insulin release by fully ionized cationic amino acids, such as L-arginine. Hence, the present findings argue against the view that the insulinotropic action of cationic amino acids is solely attributable to the accumulation of these positively charged molecules inside the islet B cell with subsequent depolarization of the plasma membrane.

• OSTI ID: 6611294
• Journal Information: Endocrinology; (USA), Journal Name: Endocrinology; (USA) Vol. 127:1; ISSN ENDOA; ISSN 0013-7227
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALKALI METAL ISOTOPES
ALKALINE EARTH METALS
AMINES
AMINO ACIDS
ANIMALS
ARGININE
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM
CARBOHYDRATES
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELEMENTS
ENDOCRINE GLANDS
GLANDS
GLUCOSE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HEXOSES
HISTAMINE
HISTIDINE
HORMONES
IMIDAZOLES
INSULIN
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METALS
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
ORNITHINE
OXYGEN
PANCREAS
PEPTIDE HORMONES
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SACCHARIDES
SECRETION
TRACER TECHNIQUES
VERTEBRATES