Stimulus-secretion coupling of arginine-induced insulin release: comparison with lysine-induced insulin secretion

Journal Article · Mon May 01 04:00:00 EDT 1989 · Endocrinology; (United States)

DOI:https://doi.org/10.1210/endo-124-5-2558· OSTI ID:6076254

Sener, A; Blachier, F; Rasschaert, J; Mourtada, A; Malaisse-Lagae, F; Malaisse, W J

L-Lysine, like-L-arginine, L-ornithine, or L-homoarginine, accumulated in rat pancreatic islets and stimulated /sup 86/Rb efflux, /sup 45/Ca uptake and efflux, and insulin release in islets exposed to D-glucose (7.0 mM). The effect of L-lysine differed from that of the other cationic amino acids by such features as the absence of a threshold concentration for stimulation of insulin release, a much lesser sensitivity of the secretory response to intracellular acidification, and the stimulation of /sup 86/Rb net uptake over 60 min of incubation. This coincided with the fact that even in the absence of another exogenous nutrient, L-lysine was well oxidized, augmented NH/sup 4 +/ production, increased both the ATP content and ATP/ADP ratio, caused a time-related decrease in /sup 86/Rb fractional outflow, and provoked either a transient (10 mM L-lysine) or sustained (20 mM L-lysine) stimulation of insulin secretion. It is proposed, therefore, that the functional response of the pancreatic B-cell to L-lysine involves not only a biophysical mechanism similar to that responsible for the insulinotropic action of L-homoarginine, but also a significant, albeit modest, metabolic component, which reflects the capacity of L-lysine to act as a fuel in islet cells.

• Research Organization: Brussels Free Univ. (Belgium)
• OSTI ID: 6076254
• Journal Information: Endocrinology; (United States), Journal Name: Endocrinology; (United States) Vol. 124:5; ISSN ENDOA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ALKALI METAL ISOTOPES
ALKALINE EARTH ISOTOPES
AMINO ACIDS
ANIMALS
ARGININE
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CALCIUM 45
CALCIUM ISOTOPES
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENDOCRINE GLANDS
EVEN-ODD NUCLEI
GLANDS
HORMONES
INSULIN
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LYSINE
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PEPTIDE HORMONES
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
RUBIDIUM 86
RUBIDIUM ISOTOPES
SECRETION
TRACER TECHNIQUES
VERTEBRATES