Ketone bodies and islet function: sup 86 Rb handling and metabolic data
- Brussels Free Univ. (Belgium)
The metabolism of ketone bodies was investigated in rat pancreatic islets incubated in the absence or presence of D-glucose. The generation of {sup 14}CO{sub 2} from 3-{sup 14}C-labeled ketone bodies, the interconversion of D-(-)-beta-hydroxybutyrate and acetoacetate (AcAc), the reciprocal effects of ketone bodies and D-glucose on their respective catabolism, and the influence of these exogenous nutrients on the output of {sup 14}CO{sub 2} from islets preincubated with either L-(U-{sup 14}C)glutamine or (U-{sup 14}C)palmitate provided an estimation of the nutrient-induced changes in O2 uptake that was in fair agreement with the observed modifications of islet respiration. There was a close correlation between such changes and the corresponding values for insulin output. Because the stimulation of insulin release by ketone bodies also coincided with a decrease in 86Rb outflow from prelabeled islets, these findings suggest that the insulinotropic action of ketone bodies is causally linked to their catabolism through an increase in ATP generation rate and a subsequent decrease in K+ conductance. A complementary participation of changes in mitochondrial redox state to stimulus-secretion coupling is considered, however, in the light of comparisons between the effects of D-(-)-beta-hydroxybutyrate and AcAc, respectively, on mitochondrial NADH generation, 45Ca net uptake, and D-(6-{sup 14}C)glucose oxidation.
- OSTI ID:
- 6873856
- Journal Information:
- American Journal of Physiology; (USA), Vol. 259; ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INSULIN
SECRETION
KETONES
CATABOLISM
PANCREAS
BIOLOGICAL FUNCTIONS
ACETOACETATES
ATP
BUTYRIC ACID
CARBON 14 COMPOUNDS
GLUCOSE
HEXADECANOIC ACID
IN VITRO
MEMBRANE TRANSPORT
MITOCHONDRIA
NADH2
OXYGEN
RATS
RESPIRATION
RUBIDIUM 86
TRACER TECHNIQUES
ALDEHYDES
ALKALI METAL ISOTOPES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
COENZYMES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELEMENTS
ENDOCRINE GLANDS
FUNCTIONS
GLANDS
HEXOSES
HORMONES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NONMETALS
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
RADIOISOTOPES
RODENTS
RUBIDIUM ISOTOPES
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques