Assignment of 112 microsatellite markers to 23 chromosome 11 subregions delineated by somatic hybrids: Comparison with the genetic map

Journal Article · Sun May 15 04:00:00 EDT 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1280· OSTI ID:6872327

Couillin, Ph; Reguigne, I ^[1]; Le Guern, E; Ravise, N ^[2]; Vignal, A; Fizames, C; Delportes, D ^[3]; Rosier, M F ^[4]; Junien, C ^[5]; Heyningen, V van ^[6]

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1. Inserm U178, Villejuif (France)
2. Inserm U289, Paris (France)
3. Genethon, Evry (France)
4. CNRS UPR420, Villejuif (France)
5. Inserm U73, Paris (France)
6. Western General Hospital, Edinburgh (United Kingdom); and others

Using a panel of 25 somatic cell hybrids, the authors have regionally localized 112 microsatellite markers generated by Genethon and assigned to chromosome 11. A genetic map of 74 of them was produced using linkage analysis of the eight largest CEPH (Centre d'Etude du Polymorphisme Humain) families. They could be ordered on chromosome 11 with an average distance of 2.1 cM. The tight correlation observed between the genetic order and the physical assignment of these microsatellites reinforces the genetic map data. These newly localized markers identified by the PCR method using a standardized protocol represent useful tools for mapping YAC clones and establishing YAC contigs and for studying genetic diseases or cancers associated with specific genes and/or germinal/somatic rearrangements of chromosome 11. 60 refs., 2 figs., 2 tabs.

• OSTI ID: 6872327
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 21:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
CHROMOSOMES
GENE AMPLIFICATION
GENETIC MAPPING
HUMAN CHROMOSOMES
MAPPING
POLYMERASE CHAIN REACTION