A microsatellite genetic linkage map of human chromosome 13

Journal Article · Fri Jan 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1993.1012· OSTI ID:6707250

Petrukhin, K E; Speer, M C; Vayanis, E; Fatima Bonaldo, M de; Soares, M B; Fischer, S G; Warburton, D ^[1]; Gilliam, C; Ott, J ^[2]; Tantravahi, U ^[3]

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1. Columbia Univ., New York, NY (United States)
2. Columbia Univ., New York, NY (United States) New York State Psychiatric Institute, New York, NY (United States)
3. Brown Univ., Providence, RI (United States)

We have characterized 21 polymorphic (CA), microsatellites for the development of a genetic map of chromosome 13. Fifteen markers were isolated from a flow- sorted chromosome 13 library, four CA repeats were derived from NotI-containing cosmid clones, and two polymorphic markers were described previously (J. L. Weber, A. E. Kwitek, and P. E. May, 1990, Nucleic Acids Res. IS: 4638; L. Warnich, 1. Groenwald, L. Laubscher, and A. E. Retief, 1991, Am. J. Hum. Genet. 49(Suppl.): 372 (Abstract)). Regional localization for all of the markers was performed by amplification of DNA from five somatic cell hybrids containing different deletions of chromosome 13. Genetic markers were shown to be distributed throughout 6 of the 11 resolvable chromosomal subregions. Using data from nine families provided by the Centre d'Etude du Polymorphisme Humain (CEPH), a framework map of 12 of these 21 markers was developed. Six of the 12 markers form three pairs, with each two members of a pair being tightly linked, such that nine systems of markers can be distinguished. The average heterozygosity of these 12 markers is 0.75. The total length of the sex-averaged map is 65.4 cM (Kosambi), with an average distance of 8.2 cM between systems of markers (eight intervals). Seven remaining markers were placed provisionally into the framework map. 41 refs., 3 figs., 4 tabs.

• OSTI ID: 6707250
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 15:1; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMPLIFICATION
BIOLOGICAL MARKERS
BIOLOGICAL VARIABILITY
CHROMOSOMES
DNA
GENETIC MAPPING
GENETIC VARIABILITY
HUMAN CHROMOSOMES
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS