Radioenzymatic assay for femtomole determination of catecholamines using. cap alpha. -methyldopamine as an internal standard

Journal Article · Mon Nov 24 04:00:00 EST 1980 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(80)90410-5· OSTI ID:6868090

Bauce, L; Thornhill, J A; Cooper, K E; Veale, W L

A radioenzymatic assay has been developed for the sensitive determination of plasma catecholamines in perchloric acid extracts using ..cap alpha..-methyldopamine as an internal standard. With 25 ..mu..l of plasma extract in a total volume of 40 ..mu..l the assay gives blank values equivalent to approximately 2 femtomoles (fmole) for epinephrine (E), norepinephrine (NE), 6 fmole for ..cap alpha..-methyldopamine (MeDA) and approximately 15 femtomoles for dopamine (DA). Recoveries of 25 dpm/fmole NE, 40 dpm/fmole E, 56 dpm/fmole DA and 80 dpm/fmole MeDA have been obtained.

• Research Organization: Univ. of Calgary, Canada
• OSTI ID: 6868090
• Journal Information: Life Sci.; (United States), Journal Name: Life Sci.; (United States) Vol. 27:21; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ADRENALINE
AMINES
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY
BODY FLUIDS
CATECHOLAMINES
DOPAMINE
DRUGS
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INORGANIC ACIDS
MATERIALS
NORADRENALINE
ORGANIC COMPOUNDS
PERCHLORIC ACID
PHENOLS
POLYPHENOLS
RADIOASSAY
STEROID HORMONES
SYMPATHOMIMETICS
TABLES
TISSUES