A highly sensitive radioenzymatic assay for simultaneous estimation of norepinephrine, dopamine, and epinephrine
Journal Article
·
· J. Pharmacol. Methods; (United States)
OSTI ID:5586850
We have developed a radioenzymatic assay for simultaneous estimation of norepinephrine (NE), dopamine (DA) and epinephrine (E) that was the result of the integration of several unique features of previously described assay procedures. Catecholamines in sera or tissue homogenates were enzymatically 0-methylated in the presence of partially purified catechol-0-methyltransferase with S-(methyl-/sup 3/H) adenosyl methionine serving as the methyl donor. The 0-methylated products were then separated by thin-layer chromatography, eluted from the gel, and their tritium content determined. The assay allows measurement of catecholamines with a sensitivity in the ranges of 15-20 pg. In addition, the assay is highly specific, reproducible, relatively rapid and simple, and inexpensive.
- Research Organization:
- Washington Univ. School of Medicine, St. Louis, MO
- OSTI ID:
- 5586850
- Journal Information:
- J. Pharmacol. Methods; (United States), Journal Name: J. Pharmacol. Methods; (United States) Vol. 3:4; ISSN JPMED
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ADRENALINE
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD SERUM
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CHEMICAL REACTIONS
DOPAMINE
DRUGS
ENZYMES
HOMOGENATES
HORMONES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
METHYLATION
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
PATIENTS
PHENOLS
POLYPHENOLS
RADIOASSAY
RATS
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
TISSUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADRENAL HORMONES
ADRENALINE
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD SERUM
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CHEMICAL REACTIONS
DOPAMINE
DRUGS
ENZYMES
HOMOGENATES
HORMONES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
METHYLATION
NEUROREGULATORS
NORADRENALINE
ORGANIC COMPOUNDS
PATIENTS
PHENOLS
POLYPHENOLS
RADIOASSAY
RATS
RODENTS
STEROID HORMONES
SYMPATHOMIMETICS
TISSUES
TRANSFERASES
TRITIUM COMPOUNDS
VERTEBRATES