Simultaneous radioenzymatic assay of dopamine and dihydroxyphenylacetic acid: an index of in vivo dopamine release
Journal Article
·
· J. Pharmacol. Methods; (United States)
The relative brain tissue concentrations of dopamine (DA) and its deaminated metabolite, dihydroxyphenylacetic acid (DOPAC), appears to be a reliable index of the functional activity of dopaminergic neurons. In order to apply this approach to the assessment of dopaminergic neuronal activity in small regions of brain, we have developed a sensitive radioenzymatic assay for simultaneous measurement of DA and DOPAC. The sensitivity of the assay for DA is approximately 10 pg and for DOPAC 100 pg. In addition, the assay is highly specific, simple, and relatively inexpensive. The concurrent estimation of tissue DA and DOPAC concentrations seems to be a reliable means of evaluating the rate of DA turnover or release in behavioral, electrical stimulation, and certain drug paradigms. However, the release or turnover of DA as induced by D-amphetamine (and perhaps other indirectly-acting dopaminemimetic drugs) cannot be meaningfully assessed by measurement of DA and DOPAC alone.
- OSTI ID:
- 5527582
- Journal Information:
- J. Pharmacol. Methods; (United States), Journal Name: J. Pharmacol. Methods; (United States) Vol. 5:2; ISSN JPMED
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BEHAVIOR
BENZEDRINE
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DOPAMINE
DRUGS
HYDROXY COMPOUNDS
KINETICS
METABOLITES
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
PHYSIOLOGY
POLYPHENOLS
RADIOASSAY
REACTION KINETICS
RECEPTORS
SENSITIVITY
SOMATIC CELLS
SPECIFICITY
SYMPATHOMIMETICS
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL CELLS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BEHAVIOR
BENZEDRINE
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DOPAMINE
DRUGS
HYDROXY COMPOUNDS
KINETICS
METABOLITES
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
PHYSIOLOGY
POLYPHENOLS
RADIOASSAY
REACTION KINETICS
RECEPTORS
SENSITIVITY
SOMATIC CELLS
SPECIFICITY
SYMPATHOMIMETICS