Radioenzymatic assay of catecholamines in plasma after a preliminary solvent extraction compared with an analogous liquid-chromatographic method

Journal Article · Mon Feb 01 04:00:00 EST 1988 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5243764

Feoli, J; Bijault, C; Dehennin, L

Catechol-borate complexation and ion-pair formation combined with organic solvent extraction as preliminaries to radioenzymatic assay (REA) with catechol-O-methyltransferase-catalyzed methylation allows the quantification of catecholamines in plasma samples of any volume. We attained improved detection limits for 1-mL samples: 9 ng/L for norepinephrine (NE), 8 ng/L for epinephrine (E), and 11 ng/L for dopamine (DA). Precision data resemble those obtained by REA for small-volume samples. Comparison with a liquid chromatography-electrochemical detection (LCEC) technique showed the two methods to be equivalent for NE and E determinations, but DA estimations were lower by REA than by LCEC.

• Research Organization: Fondation de Recherche en Hormonologie, Fresnes (France)
• OSTI ID: 5243764
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Journal Name: Clin. Chem. (Winston-Salem, N.C.); (United States) Vol. 34:2; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BUFFERS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CATECHOLAMINES
CHEMICAL REACTIONS
CHROMATOGRAPHY
COLLOIDS
COMPARATIVE EVALUATIONS
DISPERSIONS
DOPAMINE
DRUGS
EXTRACTION
GELATIN
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METHYLATION
NEUROREGULATORS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHENOLS
PHOSPHATES
PHOSPHORUS COMPOUNDS
POLYPHENOLS
PROTEINS
RADIOENZYMATIC ASSAY
RATS
RODENTS
SEPARATION PROCESSES
SOLVENT EXTRACTION
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES