Endonucleolytic incision of uvB irradiated DNA
Thesis/Dissertation
·
OSTI ID:6865474
Ultraviolet irradiation of DNA produces a variety of pyrimidine damages including pyrimidine dimers and 6-4' (pyrimidin-2' one)pyrimidines. Photoalkylation, the ultraviolet irradiation of DNA in the presence of isopropanol and a free radical photoinitiator had been shown to produce 8-(2-hydroxy-2-propyl)purines. An endonuclease activity against photoalkylated DNA was found in E. coli. This was assayed by conversion of photoalkylated superhelical PM2 phage DNA to the nicked form. Comparison of enzyme activities between crude extracts of E. coli strains demonstrated a deficiency associated with a mutant lacking endonuclease III. Irradiation of the DNA substrate in the absence of isopropanol did not affect the activity in any strain studied. Therefore, the substrate photoproduct for endonuclease III is not an 8-(2-hydroxy-2-propyl)purine.
- Research Organization:
- Temple Univ., Philadelphia, PA (USA)
- OSTI ID:
- 6865474
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RADICALS
SUBSTRATES
ULTRAVIOLET RADIATION
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESCHERICHIA COLI
ESTERASES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HYDROLASES
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
RADIATION EFFECTS
RADIATIONS
RADICALS
SUBSTRATES
ULTRAVIOLET RADIATION