Endonucleolytic incision of UVB-irradiated DNA
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6122150
Ultraviolet irradiation of DNA produces a variety of pyrimidine damages. In addition to the well-known photoproducts, pyrimidine dimers and 6-4'-(pyrimidin-2'one)pyrimidines, DNA sequence analysis has shown that purified E. coli endonuclease III incises broad-spectrum UV-irradiated DNA at cytosines and OsO/sub 4/-oxidized DNA at thymines. The wavelength dependence of endonuclease III incision of superhelical DNA irradiated by 250 to 320 nm. monochromatic light was examined. Maximal enzymic cleavage was obtained with substrates irradiated between 265 and 285 nm, with peak activity at 280 nm. Incision of DNA irradiated at 254 nm was reduced. Therefore, these cytosine photoproducts are optimally formed at biologically significant wavelengths and may be involved in actinic carcinogenesis. Enzymic release of modified labeled cytosine into the ethanol-soluble fraction from irradiated poly(dG-dC):poly(dG-dC) was linear with time of reaction, quantity of enzyme protein and exposure of substrate to irradiation. Identification of the cytosine UV photoproduct is currently under investigation.
- Research Organization:
- Temple Univ. Medical School, Philadelphia, PA
- OSTI ID:
- 6122150
- Report Number(s):
- CONF-870644-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 46:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CYTOSINE
DNA
DNA REPAIR
DNA SEQUENCING
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
STRUCTURAL CHEMICAL ANALYSIS
THYMINE
ULTRAVIOLET RADIATION
URACILS
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
CHEMISTRY
CYTOSINE
DNA
DNA REPAIR
DNA SEQUENCING
DNA-ASE
ELECTROMAGNETIC RADIATION
ENDONUCLEASES
ENZYME ACTIVITY
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
PHOSPHODIESTERASES
PYRIMIDINE DIMERS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
STRUCTURAL CHEMICAL ANALYSIS
THYMINE
ULTRAVIOLET RADIATION
URACILS