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Modifications of circular DNA by photoalkylation

Conference · · Radiat. Res.; (United States)
OSTI ID:5265672
The effects of photoalkylation on superhelical PM2 DNA were examined. The chief product was 8-(2-hydroxy-2-propyl)guanine, formed exclusively in sequences of alternating purines and pyrimidines. Other purine damages included 8-(2-hydroxy-2-propyl)adenine and smaller quantities of two uncharacterized adenine products. DNA strand breaks were formed with increasing irradiation. A small quantity of thymine-containing photodimers was formed. Photoalkylation of poly(dG-dC):poly(dG-dC) reduced the concentration of salt required to effect inversion of the circular dichroic spectrum. This suggests that photoalkylation induces the transition of poly(dG-dC):poly(dg-dC) from the right-handed B form of DNA to the left-handed Z form.
Research Organization:
Temple Univ. School of Medicine, Philadelphia, PA
OSTI ID:
5265672
Report Number(s):
CONF-860423-
Conference Information:
Journal Name: Radiat. Res.; (United States) Journal Volume: 103:1
Country of Publication:
United States
Language:
English