Age related decline in the proliferative response of human T cells to OKT3 stimulation
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6859583
The level of the in vitro proliferative response of human peripheral blood mononuclear cells (PBMC) to the OKT3 monoclonal antibody is directly related to the level of monocyte representation in the cell population. The responses to OKT3 stimulation of PBMC obtained from different individual are difficult to interpret due to variable percentage representation of monocytes. To eliminate this problem purified T cells from humans of various ages were incubated with 2 ng/ml OKT3 antibody and 10% purified autologous monocytes. The /sup 3/H-TdR incorporation of 1 x 10/sup 5/ T cells at 72 hrs of culture was 69,939 +/- 6085 (SEM) cpm for young individuals (mean age 35 yrs) and 33,163 +/- 2962 cpm for healthy elderly individuals (mean age 78 yrs). In addition, IL2 receptors were measured using two color fluorescence and flow cytometry with phycoerythrin conjugated anti-IL2 receptor antibody and FITC conjugated OKT11 antibody. The percentage of cells expressing IL2 receptors was 46% for the cells from the young individuals and 23% for cells from old individuals. These results suggest that the age related decline in the proliferative ability of T cells is partially due to a decreased expression of IL2 receptors and that proliferation and IL2 receptor expression is under the control of monocyte accessory cells.
- Research Organization:
- National Institute of Health, Baltimore, MD
- OSTI ID:
- 6859583
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANIMAL CELLS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MONOCLONAL ANTIBODIES
MONOCYTES
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
ANIMAL CELLS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MONOCLONAL ANTIBODIES
MONOCYTES
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS