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Alveolar macrophages are better accessory cells for OKT3-induced T cell proliferation than peripheral blood monocytes

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5326142
; ;
The accessory cell function of purified alveolar macrophages (MP) was compared with that of peripheral blood monocytes (MN) by assaying the ability of each to support the OKT3 antibody induced proliferation of purified T cells (TC). TC (1 x 10/sup 5/) co-cultured with 0.3 to 8 x 10/sup 4/ autologous MP or MN were exposed to 5 ng/ml of OKT3 antibody for 64 hrs, with /sup 3/H-thymidine present for the last 16 hrs of culture. TC, MN or MP cultured alone with OKT3 antibody resulted in /sup 3/HT incorporation of < 900 cpms. TC + 10% MP cultured with OKT3 antibody gave 3600 to 41,990 cpm which was 2 to 4 fold better than the level seen with cultures of TC + 10% MN + OKT3. The addition of increasing numbers of MN to TC + OKT3 cultures could increase the level of proliferation to that found with MP + TC + OKT3. TC + 10% MP + OKT3 cultures contained 1.5 to 3 fold more IL2 receptor positive cells and demonstrated greater IL2 production than did cultures of TC + 10% MN + OKT3. Therefore, on a cell to cell comparison, MP are much more active than MN in accessory cell function in supporting TC proliferation, induction of IL2 receptors and IL2 production.
Research Organization:
Johns Hopkins Univ. School of Medicine, Baltimore, MD
OSTI ID:
5326142
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
MITOGENS
MONOCYTES
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RECEPTORS
RIBOSIDES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS