Inhibition of OKT3 induced T4 and T8 cell proliferation by anti-Ia and anti-LFA-1 antibodies

Conference · Tue Mar 04 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7024882

Geppert, T D; Lipsky, P E

Monoclonal antibodies (Mab) directed at the CD3 molecule induce accessory cell (AC) dependent T cell proliferation. The role of AC in anti-CD3 induced proliferation is unclear but involves more than Fc receptor mediated crosslinking since Fc receptor (-) endothelial cells (EC) are effective AC for OKT3 induced proliferation. The role of AC in anti-CD3 induced proliferation was investigated by examining the ability of Mab directed at T cell and AC surface molecules to block OKT3 induced proliferation. AC-depleted T4 or T8 cells were used as responders. Monocytes (M phi), or EC, were used as AC. The Mab used included OKT4 and OKT8, 60.3, directed at the B subunit of LFA-1, MO-1 and the p150,95 protein, and L243, an anti-HLA-DR Mab. M phi-supported OKT3-induced proliferation was inhibited by 60.3, L243, and OKT4 but not OKT8. M phi-supported OKT3-induced proliferation of T8 cells was inhibited by OKT8, 60.3, and L243 but not OKT4. Much of the inhibition of T4 and T8 cell proliferation could be reversed by IL-2. L243 did not inhibit OKT3-stimulated T4 cell proliferation supported by Ia(-) EC. Moreover, neither L243 nor 60.3 inhibited AC independent stimulation of T4 cells by the combination of phorbol myristate acetate (PMA) and OKT3 or calcium ionophore and PMA. Thus, M phi support of OKT3-induced T8 and T4 cell proliferation involves recognition of Ia and an interaction involving the molecule identified by 60.3. These interactions may be necessary to promote optimal IL-2 production and thus maximal proliferation.

• Research Organization: UTHSCD, Dallas, TX
• OSTI ID: 7024882
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METALS
ANIMAL CELLS
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM
CARCINOGENS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
ELEMENTS
ESTERS
GLOBULINS
GROWTH FACTORS
IMMUNOGLOBULINS
INHIBITION
KINETICS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
METALS
MITOGENS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PHORBOL ESTERS
PROTEINS
REACTION KINETICS
RECEPTORS
SOMATIC CELLS