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Cyclosporine inhibits expression of receptors for interleukin 2 and transferrin on mitogen-activated human T lymphocytes

Journal Article · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6763707
;
Cyclosporine (CyS) has been shown to inhibit activation of human T lymphocytes. A cascade of interactions involving Interleukin 2 (IL2), Interleukin 2 receptor (IL2R) and transferrin receptor (TR) is necessary for activation. In studies using peripheral blood mononuclear cells obtained from healthy donors, the authors measured the expression of IL2 and TR (using receptor-specific monoclonal antibodies and flow cytometry) and DNA synthesis (/sup 3/-thymidine incorporation) in response to PHA, OKT3, Leu 4 or Con A. In the presence of CyS (0.5 ..mu..g/ml) expression of IL2R and TR as well as DNA synthesis were markedly reduced. Upon serial dilutions of CyS, changes in DNA synthesis in response to OKT3 reflected changes in IL2R and TR levels, indicating all 3 parameters may be interrelated. Addition of exogenous IL2 partially abrogated the inhibitory effect of CyS on these activation parameters in response to PHA, OKT3 and Leu 4 but not to Con A. The authors results suggest that CyS is an effective inhibitor of mitogen-induced expression of IL2R and TR and that exogenous IL2 partially reverses this inhibitory effect.
Research Organization:
American Red Cross Blood Services, Los Alamos, CA
OSTI ID:
6763707
Report Number(s):
CONF-8604222-
Journal Information:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:4; ISSN FEPRA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DNA REPLICATION
FUNGI
GLOBULINS
GLOBULINS-BETA
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
LYMPHOKINES
MATERIALS
MEMBRANE PROTEINS
METABOLITES
METALLOPROTEINS
MITOGENS
MONOCLONAL ANTIBODIES
MONOCYTES
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
PROTEINS
PYRIMIDINES
RECEPTORS
RIBOSIDES
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TRANSFERRIN
TRITIUM COMPOUNDS