Ketone-body utilization by homogenates of adult rat brain
The regulation of ketone-body metabolism and the quantitative importance of ketone bodies as lipid precursors in adult rat brain has been studied in vitro. Utilization of ketone bodies and of pyruvate by homogenates of adult rat brain was measured and the distribution of /sup 14/C from (3-/sup 14/C)ketone bodies among the metabolic products was analysed. The rate of ketone-body utilization was maximal in the presence of added Krebs-cycle intermediates and uncouplers of oxidative phosphorylation. The consumption of acetoacetate was faster than that of D-3-hydroxybutyrate, whereas, pyruvate produced twice as much acetyl-CoA as acetoacetate under optimal conditions. Millimolar concentrations of ATP in the presence of uncoupler lowered the consumption of ketone bodies but not of pyruvate. Indirect evidence is presented suggesting that ATP interferes specifically with the mitochondrial uptake of ketone bodies. Interconversion of ketone bodies and the accumulation of acid-soluble intermediates (mainly citrate and glutamate) accounted for the major part of ketone-body utilization, whereas only a small part was oxidized to CO/sub 2/. Ketone bodies were not incorporated into lipids or protein. We conclude that adult rat-brain homogenates use ketone bodies exclusively for oxidative purposes.
- Research Organization:
- Laboratory of Veterinary Biochemistry State University of Utrecht, Netherlands
- OSTI ID:
- 6857564
- Journal Information:
- Neurochem. Res.; (United States), Vol. 7:6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BRAIN
KETONES
METABOLISM
ACETOACETATES
ATP
BUTYRIC ACID
CARBON 14 COMPOUNDS
CARBON DIOXIDE
COENZYMES
LIPIDS
MITOCHONDRIA
OXIDATION
PHOSPHORYLATION
PYRUVIC ACID
RATS
TRACER TECHNIQUES
ANIMALS
BODY
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHALCOGENIDES
CHEMICAL REACTIONS
ISOTOPE APPLICATIONS
KETO ACIDS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques