Antigen-specific immune-suppressor factor in herpes simplex virus type 2 infections of UV B-irradiated mice
UV B-irradiation (280 to 320 nm) of mice at the site of cutaneous infection with herpes simplex virus type 2 (HSV-2) induced suppressor T-cell circuits that decreased HSV-2-induced proliferative responses of HSV-2-immune lymph node cells. Adoptive transfer experiments indicated that splenocytes from UV B-irradiated HSV-2-infected animals contain L3T4+ cells that suppress proliferative responses in vivo, consistent with suppressor inducer cells. However, following in vitro culture of the splenocytes with HSV-2 antigen, the proliferation of immune lymph node cells was inhibited by Lyt2+ suppressor T cells, consistent with antigen-induced suppressor effector cells. Antigen-specific and nonspecific suppressor factors were fractionated from supernatants of HSV-2-stimulated spleen cells by molecular-sieve chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the Sephadex fraction that contained the antigen-specific suppressor factor, in the presence or absence of 2-mercaptoethanol, defined a 115-kilodalton protein consisting of two disulfide-bound components with molecular sizes of 70 and 52 kilodaltons. The implications of these results with respect to the regulation of HSV-induced cell-mediated immunity following UV B-irradiation are discussed.
- Research Organization:
- Univ. of Maryland School of Medicine, Baltimore (USA)
- OSTI ID:
- 6856410
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:7; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Induction of suppression of delayed type hypersensitivity to herpes simplex virus by epidermal cells exposed to UV-irradiated urocanic acid in vivo
Two phenotypically distinct T cells are involved in ultraviolet-irradiated urocanic acid-induced suppression of the efferent delayed-type hypersensitivity response to herpes simplex virus, type 1 in vivo
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL PROLIFERATION
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
HERPES SIMPLEX
IMMUNITY
INFECTIOUS DISEASES
INHIBITION
LEUKOCYTES
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
RADIATIONS
RADIOINDUCTION
RODENTS
SEPARATION PROCESSES
SKIN DISEASES
SOMATIC CELLS
SPLEEN CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES