Radioimmunoassay of digoxin in serum using monoclonal antibodies and assessment of interference by digoxin-like immunoreactive substances

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Therapeutic Drug Monitoring; (USA)

DOI:https://doi.org/10.1097/00007691-199003000-00015· OSTI ID:6855162

Loucari-Yiannakou, E; Yiannakou, L; Souvatzoglou, A; Diamandis, E P ^[1]

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1. Alexandra General Hospital, Athens (Greece)

We used 7 monoclonal antibodies (MoAbs) and one polyclonal antibody to develop radioimmunoassays (RIAs) for digoxin in serum or plasma. These RIAs were tested for measuring apparent digoxin concentrations in serum from patients receiving the drug, from normal individuals, and in cord blood plasma. We found that two MoAbs cross-reacted significantly with substances in cord blood. The magnitude of cross-reactivity was dependent on the incubation time and temperature. Under equilibrium conditions, one antibody gave apparent digoxin values in cord blood plasma averaging 2.15 ng/ml. We suggest that this cross-reactivity is partially due to progesterone and 17-hydroxyprogesterone in cord blood plasma. The antibody that shows high cross-reactivity with digoxin-like immunoreactive substances may prove a useful tool for studies dealing with characterization of the cross-reacting compounds.

• OSTI ID: 6855162
• Journal Information: Therapeutic Drug Monitoring; (USA), Journal Name: Therapeutic Drug Monitoring; (USA) Vol. 12:2; ISSN 0163-4356; ISSN TDMOD
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BIOASSAY
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
DIAGNOSTIC TECHNIQUES
DIGITALIS GLYCOSIDES
DIGOXIN
DRUGS
FETUSES
GLYCOSIDES
HORMONES
IMMUNOASSAY
IMMUNOLOGY
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
MAN
MATERIALS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PREGNANES
PRIMATES
PROGESTERONE
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
STEROID HORMONES
STEROIDS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TRACER TECHNIQUES
VERTEBRATES