Sequential assessment of pulmonary epithelial diethylene triamine penta-acetate clearance and intrapulmonary transferrin accumulation during Escherichia coli peritonitis

Journal Article · Wed Feb 28 23:00:00 EST 1990 · American Review of Respiratory Disease (New York); (USA)

DOI:https://doi.org/10.1164/ajrccm/141.3.631· OSTI ID:6854702

Ishizaka, A; Stephens, K E; Segall, G M; Hatherill, J R; McDougall, I R; Wu, Z; Raffin, T A ^[1]

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1. Stanford Univ. Medical Center, CA (USA)

The individual roles of pulmonary capillary endothelial and alveolar epithelial permeability in the pathogenesis of the adult respiratory distress syndrome (ARDS) are unclear. We developed a method for the sequential assessment of pulmonary macromolecule accumulation and small solute clearance in vivo using a gamma camera. We measured the exponential clearance coefficient of 111In-labeled diethylene triamine penta-acetate (111In-DTPA) to assess airway clearance of small solutes. We also calculated the exponential equilibration coefficient of 111In-labeled transferrin (111In-TF) to assess intrapulmonary accumulation of transferrin. We determined these parameters in guinea pigs with Escherichia coli peritonitis and compared them with a saline-treated control group, oleic-acid-treated groups, and a group treated with low molecular weight dextran Ringer solution. The pulmonary DTPA clearance and the intrapulmonary transferrin accumulation were significantly increased in the peritonitis group (29.4 +/- 8.2 x 10(-3) min-1, p less than 0.02, and 15.1 +/- 3.1 x 10(-3) min-1, p less than 0.02) when compared with the control group (3.1 +/- 0.8 x 10(-3) min-1 and 4.5 +/- 0.5 x 10(-3) min-1). These changes developed within 5.5 h of the initial insult. Neither increased extravascular lung water nor elevated pulmonary artery and left atrial pressures were detected in the peritonitis group. The low molecular weight dextran Ringer group did not show a significant increase in the pulmonary DTPA clearance and the intrapulmonary transferrin accumulation.

• OSTI ID: 6854702
• Journal Information: American Review of Respiratory Disease (New York); (USA), Journal Name: American Review of Respiratory Disease (New York); (USA) Vol. 141:3; ISSN ARDSB; ISSN 0003-0805
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CAMERAS
CAPILLARIES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CELL MEMBRANES
CHELATING AGENTS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
EPITHELIUM
ESCHERICHIA COLI
EXCRETION
GAMMA CAMERAS
GLOBULINS
GLOBULINS-BETA
GUINEA PIGS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LAVAGE
LUNG CLEARANCE
LUNGS
MAMMALS
MEMBRANES
METALLOPROTEINS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-EVEN NUCLEI
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PERMEABILITY
PROTEINS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RESPIRATORY SYSTEM
RESPIRATORY SYSTEM DISEASES
RODENTS
TISSUES
TRANSFERRIN
VERTEBRATES