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Detection of diffuse glomerular lesions in rats: II. Comparison of indium-111 cationic small macromolecules with technetium-99m DTPA

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:5327637
; ; ; ; ; ; ;

Dextrans with average molecular weights of 5000, 10,000, and 17,500 and inulin were rendered cationic by amination with 2-bromoethylamine hydrobromide. After limited coupling with DTPA cyclic dianhydride, they were labeled with 111In. A good correlation was found between their early renal uptake quantitated by camera-computer techniques and their renal clearance from multiple plasma samples in rats with glomerular damage induced by puromycin aminonucleoside and controls. However, there was poor correlation between the early renal uptake of these agents and the clearance of simultaneously injected (/sup 99m/Tc)DTPA. The 2-hr organ distribution and urinary excretion of these agents were compared with the corresponding values of DTPA. The differences in clearance between rats with glomerular damage and controls were greater with aminated dextran (mol wt 5000) than with DTPA, confirming previous work with infusions of nonradioactive charged dextrans and neutral inulin. The cationic dextrans appear to reflect the presence or absence of the normal anionic charge of the glomerular membrane as well as changes in filtration rate. Aminated inulin did not differentiate between controls and rats with glomerular disease any better than DTPA, probably because the number of amino groups conjugated was insufficient to produce the charge effect.

Research Organization:
Upstate Medical Center, Syracuse, NY
OSTI ID:
5327637
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 4; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD SUBSTITUTES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
CLEARANCE
DAYS LIVING RADIOISOTOPES
DEXTRAN
DRUGS
DTPA
ELECTRON CAPTURE RADIOISOTOPES
EXCRETION
HEMATOLOGIC AGENTS
HOURS LIVING RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
INULIN
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLING
MAMMALS
MINUTES LIVING RADIOISOTOPES
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RATS
RENAL CLEARANCE
RODENTS
SACCHARIDES
TECHNETIUM 99
TECHNETIUM ISOTOPES
TRACER TECHNIQUES
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES