Ozone effects on mechanics and arachidonic acid metabolite concentrations in isolated rat lungs

Journal Article · Mon Aug 01 00:00:00 EDT 1994 · Environmental Research; (United States)

DOI:https://doi.org/10.1006/enrs.1994.1054· OSTI ID:6853765

Joad, J P; McDonald, R J; Giri, S N; Bric, J M ^[1]

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1. Univ. of California, Davis, CA (United States)

In whole animals, ozone acutely impairs pulmonary function, an effect which may be mediated by eicosanoids. This study determined if the lung itself, separated from blood components and the CNS, responds to ozone with increased eicosanoid production and decreased function. Wistar rat lungs were placed in a negatively ventilated, buffer-perfused, isolated system, where they were exposed to filtered air or 1 ppm ozone for 3 hr. ozone increased lung resistance (P = 0.02) and decreased dynamic compliance (P = 0.003, multivariate ANOVA for repeated measures). Pulmonary artery pressure, lung weight/body weight, and cells in lung lavage (BALF) did not change. In the perfusate, ozone increased the concentration of 6-keto-PGF[sub 1[alpha]]; PGF[sub 2[alpha]]; PGD[sub 2], and TxB[sub 2] (all P < 0.05, t test) but not LTC[sub 4]/D[sub 4] or PGE[sub 2]. In BALF, ozone did not change the concentrations of any of the arachidonic acid metabolites tested (PGE[sub 2], 6-keto-PGF[sub 1[alpha]], PGD[sub 2], or LTC[sub 4]/D[sub 4]). We conclude that ozone increase intravascular concentrations of some cyclooxygenase products in the isolated buffer-perfused lung, an effect associated with reduced lung function. 36 ref., 3 figs., 2 tabs.

• OSTI ID: 6853765
• Journal Information: Environmental Research; (United States), Journal Name: Environmental Research; (United States) Vol. 66:2; ISSN ENVRAL; ISSN 0013-9351
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
560300 -- Chemicals Metabolism &amp; Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CHEMICAL ACTIVATION
EICOSANOIC ACID
LUNGS
MAMMALS
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OZONE
RATS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES