The role of arachidonic acid metabolism in virus-induced alveolar macrophage dysfunction

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1988

OSTI ID:7168125

Laegreid, W W

Alveolar macrophages (AM) recovered from virus-infected lungs have decreased phagocytic, respiratory burst and bactericidal activities. The studies described below investigated the role of eicosanoids in virus induced AM bactericidal dysfunction. The spectrum of eicosanoid metabolites which bovine AM are capable of producing was determined. Cultured AM were exposed to {sup 3}H-arachidonate for 1 hour, stimulated for 4 hours with A23187, phorbol myristate acetate or zymosan and the supernatants extracted and analyzed by HPLC. All stimuli tested caused the release of these cyclooxygenase metabolites: thromboxane B{sub 2}, PGF{sub 2}, PGE{sub 2}, PGD{sub 2} and HHT. The effect of this enhanced release of arachidonate metabolites on the ability of AM to kill bacteria was evaluated. Preincubation with cyclooxygenase inhibitors or dual cyclooxygenase and lipoxygenase inhibitors resulted in partial reversal of the virus-induced bactericidal deficit in PI3 infected AM.

• Research Organization: Washington State Univ., Pullman, WA (USA)
• OSTI ID: 7168125
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ARACHIDONIC ACID
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
BODY
CARBOXYLIC ACIDS
CARCINOGENS
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DISEASES
ENZYME INHIBITORS
ENZYMES
ESTERS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LIQUID COLUMN CHROMATOGRAPHY
LUNGS
MACROPHAGES
METABOLISM
METABOLITES
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PARASITES
PHAGOCYTES
PHORBOL ESTERS
RESPIRATORY SYSTEM
SENSITIVITY
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VIRAL DISEASES
VIRUSES
ZYMOSAN