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Modulation of arachidonic acid metabolism by bovine alveolar macrophages

Thesis/Dissertation ·
OSTI ID:6827684

The purpose of this study were to identify the arachidonic acid (AA) metabolites produced by cultured bovine alveolar macrophages (AM), to investigate the effects of various stimuli on the production of those metabolites, and to study the effect of interferons and lipopolysaccharide on AA metabolism by AM. Initial studies were conducted to ascertain which AA metabolites are produced by bovine alveolar macrophages. Cultured macrophages were labeled with tritiated arachidonic acid and stimulated with calcium ionophore A23187. The radiolabeled AA metabolites released were identified using reverse-phase high-performance liquid chromatography. The production of LTB{sub 4}, TXB{sub 2}, and PGF{sub 2{alpha}} by AM stimulated with A23187 or opsonized zymosan (OPZ) was measured using radioimmunoassay. Finally, the effects of recombinant bovine interferon alpha{sub 1}-1 (IFN-{alpha}{sub 1}-1), recombinant bovine interferon gamma (IFN-{gamma}), and lipopolysaccharide (LPS) derived from Escherichia coli 0111:B4 on the AA metabolism of bovine AM were investigated. These studies indicate that appropriately stimulated bovine AM are the source of a number of AA metabolites. Furthermore, the production of these metabolites may be dramatically altered by exposure of the AM to IFNs or LPS. Such exposure could occur in vivo during gram negative bacterial pneumonias following viral infections. Because AA metabolites are intimately involved in the inflammatory process, it is possible that AM may contribute to the development of pulmonary inflammation in certain situations.

Research Organization:
North Carolina State Univ., Raleigh, NC (USA)
OSTI ID:
6827684
Country of Publication:
United States
Language:
English

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Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ARACHIDONIC ACID
BACTERIA
BIOASSAY
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CATTLE
CHROMATOGRAPHY
CONNECTIVE TISSUE CELLS
DIAGNOSTIC TECHNIQUES
DISEASES
DOMESTIC ANIMALS
ESCHERICHIA COLI
FUNCTIONS
GROWTH FACTORS
HYDROGEN COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
INTERFERON
ISOTOPE APPLICATIONS
KINETICS
LIQUID COLUMN CHROMATOGRAPHY
LYMPHOKINES
MACROPHAGES
MAMMALS
METABOLISM
METABOLITES
MICROORGANISMS
MITOGENS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RESPIRATORY SYSTEM DISEASES
RUMINANTS
SEPARATION PROCESSES
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
ZYMOSAN