Thromboxane and prostacyclin synthesis following whole body irradiation in rats

Journal Article · Sat Dec 31 23:00:00 EST 1983 · J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States)

OSTI ID:5751607

Schneidkraut, M J; Kot, P A; Ramwell, P W; Rose, J C

The effect of radiation on the mechanism and source of in vivo thromboxane B/sub 2/ (TxB/sub 2/) and 6-keto-prostaglandin F/sub 1..cap alpha../ (6-keto-PGF/sub 1..cap alpha..) synthesis was evaluated. Rats were irradiated with 2, 10, or 20 gray (Gy) whole body gamma irradiation and showed an increase in urine TxB/sup 2/ after either 10 or 20 Gy. Urine 6-keto-PGF/sub 1..cap alpha../ was elevated only after exposure to 20 Gy. Irradiation did not alter urine volume and osmolarity, nor was there a correlation between urine osmolarity and the urinary concentration of TxB/sup 2/ or 6-keto-PGF/sub 1..cap alpha../. Rats were pretreated with indomethacin to determine if radiation-induced alterations in urine TxB/sup 2/ and 6-keto-PGF/sub 1..cap alpha../ could be suppressed. Pretreatment with indomethacin significantly decreased urine TxB..cap alpha.. and 6-keto-PFG/sub 1..cap alpha../ in both irradiated and nonirradiated animals. Finally, the sources of urinary cyclooxygenase products were investigated using an isogravitometric cross-perfusion system. These experiments demonstrated that urine TxB..cap alpha.. is derived from extrarenal sources, whereas 6-keto-PGF/sub 1..cap alpha.. is synthesized primarily by the kidney. It may be concluded that radiation exposure increases in vivo cyclooxygenase pathway activity by both renal and ultrarenal tissues.

• Research Organization: Georgetown Univ. Medical Center, Washington, DC
• OSTI ID: 5751607
• Journal Information: J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States), Journal Name: J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States) Vol. 57:3; ISSN JARPD
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL WASTES
BIOSYNTHESIS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DOSES
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
EVEN-ODD NUCLEI
EXTERNAL IRRADIATION
GAMMA RADIATION
IN VIVO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KIDNEYS
MAMMALS
MATERIALS
NUCLEI
ORGANS
OXIDOREDUCTASES
OXYGENASES
PROSTAGLANDINS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RATS
RODENTS
SYNTHESIS
URINE
VERTEBRATES
WASTES
WHOLE-BODY IRRADIATION
XENON 133
XENON ISOTOPES