Suppression of male pronuclear movement in frog eggs by hydrostatic pressure and deuterium oxide yields androgenetic haploids
The disruptive effects of microtubule-specific agents on pronuclear movement illustrate the requirement of an intact cytoskeletal system for movement. In this study, we investigated the effects of high hydrostatic pressure and deuterium oxide (D/sub 2/O) on fertilized Rana pipiens eggs during the time of pronuclear migration. The eggs were either pulsed for six min with 3000, 5000, or 7000 psi or placed for ten min in 80% D/sub 2/O between the time of second polar body emission and first cleavage. Both treatments disrupted male pronuclear migration as shown by eccentric first cleavage furrows. Treatment of eggs prior to pronuclear association resulted in haploid production. The androgenetic origin of the haploid embryos was demonstrated using morphological and isozymal markers produced by the cross Rana pipiens female x Rana utricularia male. Eggs treated with D/sub 2/O also yielded embryos with neural defects identical to those following ultraviolet irradiation. This study complements the recent reports on pressure-suppression of the second polar body and of first cleavage by showing that the selective suppression of microtubular function between these two events produces an entirely different set of genetic and developmental consequences.
- Research Organization:
- Department of Zoology, University of Toronto, Ontario, Canada
- OSTI ID:
- 6853130
- Journal Information:
- J. Exp. Zool.; (United States), Journal Name: J. Exp. Zool.; (United States) Vol. 222:1; ISSN JEZOA
- Country of Publication:
- United States
- Language:
- English
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560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
CELL CONSTITUENTS
CELL DIVISION
FROGS
GAMETES
GERM CELLS
HEAVY WATER
HYDROGEN COMPOUNDS
MORPHOLOGICAL CHANGES
OVA
OXYGEN COMPOUNDS
PRESSURIZING
TERATOGENESIS
VERTEBRATES
WATER